GrainGenes Marker Report: Stb12
Locus
Stb12
Type
Trait
Chromosome
4A
Chromosome Arm
4AL
Map
Wheat-Composite2004-4A 140
[ Show Nearby Loci ]
Species
Triticum aestivum
Probe
WMC219
Associated Gene
Stb12 (Triticum)
Gene Class
Reaction to Mycosphaerella graminicola (Fuckel) Schroeter
Reference
Chartrain L et al. (2005) Resistance of wheat line Kavkaz-K4500 l.6.A.4 to Septoria triici blotch controlled by isolate-specific resistance genes. Phytopathology 95:664-671.
Remark
Trait marker Xwmc219-4A Nearby marker on Composite map: Xwmc219 Position on Composite map estimated by GrainGenes.
Probe for Locus
WMC219
Locus
[ Hide all but 1 of 7 ] Xwmc219-4A Xwmc219 Stb12 Yr60 SrND643 wmc219 wmc219-4A
Reference
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 11b9 Donor: Benoist AGR205.18. Overlap of forward and reverse is hypothetical - both end within a substantial micorsatellite. The fragment sizes for the PCR from this sequence will not match the expected size. Trimmed to Rsa1 at both ends. (CA)57 316 to 429.
Type
PCR SSR
PCR primers
Forward TgcTAgTTTgTcATccgggcgA at 264 length 22 bases, Tm 71
Reverse cAATcccgTTcTAcAAgTTccA at 446 length 22 bases, Tm 63
SSR size
~204 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence
WMC219
Source Gene
Yr60 (Triticum)
Source Species
Triticum aestivum
GrainGenes Marker Report: Stb12
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