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GrainGenes Marker Report: Tg1

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Locus	Tg1
Type	Trait
Chromosome	2D
Chromosome Arm	2DS
Map	Wheat-Composite2004-2D 27
	[ Show Nearby Loci ]
Species	Triticum aestivum
Probe	WMC112
Associated Gene	Tg1 (Triticum)
Gene Class	Tenacious glumes
Reference	Nalam VJ et al. (2007) Map-based analysis of genetic loci on chromosome 2D that affect glume tenacity and threshability, components of the free-threshing habit in common wheat (Triticum aestivum L.). Theoretical and Applied Genetics 116:135.
Remark	Trait marker Xwmc112-2D, Xbarc168-2D Nearby marker on Composite map: Xwmc112y, Xbarc168 Position on Composite map estimated by GrainGenes.
Probe for Locus	WMC112
Locus	[ Hide all but 1 of 6 ] Xwmc112-2D Xwmc112 Xwmc112y Tg1 wmc112 wmc112-2D
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 8g8 Donor: Flore, Verneuil Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 251 bases located on 5B AGR170. Trimmed to Rsa1 at both ends. (CA)17 202 to 235, (CT)34 134 to 201. Good clear polymorphism (1 locus).
Type	PCR SSR
PCR primers	Forward TgAgTTgTggggTcTTgTTTgg at 105 length 22 bases, Tm 66.0 Reverse TgAAggAgggcAcATATcgTTg at 328 length 22 bases, Tm 66.0
SSR size	245 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC112
Source Species	Triticum aestivum