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GrainGenes Marker Report: WMC157

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Probe	WMC157
Locus	[ Hide all but 1 of 6 ] Xwmc157-7D Xwmc157 Gbd Gbz Gbx1 wmc157
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11c5 Donor: Rogers, Monsanto AGR204.35.SEQ. Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)29 212 to 269. Redesign primers? Bases 115-229 (ie 5' side of microsatellite) match part of 5g9. Bases 189 to 263 show homology to 24c1 bases 82 to 156 (75 in longest run, maxScore = 82), duplicate, 11c5 has precedence.
Type	PCR SSR
PCR primers	Forward cTTgATccAAgTggTTcTTTcc at 183 length 22 bases, Tm 62 Reverse TccAAATgTTTgcgAAAccTgA at 270 length 22 bases, Tm 66
SSR size	109 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear; 51C anneal, product;
Sequence	WMC157
Source Species	Triticum aestivum