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GrainGenes Marker Report: WMC175

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Probe	WMC175
Locus	[ Hide all but 1 of 13 ] Xwmc175-2B Xwmc175-3A Xwmc175 Xwmc175b Xwmc175a Xwmc175c Xwmc175-2D wmc175 wmc175-2B wmc175A wmc175-2B.1 wmc175-2B.2 wmc175-2D
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 9b4 Donor: Bernard, Clermont-Ferrand Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 135 bases located on 2B, 145 bases located on 2B, 181 bases located on 2B, 256 bases located on 2B Trimmed to insert at 5' end and to Rsa1 at 3' end. (CA)16 138 to 169.
Type	PCR SSR
PCR primers	Forward gcTcAgTcAAAccgcTAcTTcT at 17 length 22 bases, Tm 62 Reverse cAcTAcTccAATcTATcgccgT at 248 length 22 bases, Tm 62
SSR size	253 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC175
Source Species	Triticum aestivum