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GrainGenes Marker Report: WMC1E8

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Locus	WMC1E8
Type	Microsatellite
Chromosome	1H
Map	[ Hide all but 1 of 16 ] Barley, B73xCPI-1H 127 Barley-ConsensusFHB-1H 146 Hordeum-Consensus2006-DArT-1H 143.2 Hordeum-Consensus2007-SSR-1H 131.93 Hordeum-LiHs-SNP-1H 157.97 Hordeum-LxHs-1H 164 Hordeum-OWB-1H 130.3 Hordeum-OWB-2004-1H 133.7 Hordeum-OWB-2005-1H 129.6 Hordeum-OWB-OPA2008-1H 150.815 Hordeum-OWB-SNP-1H 130.41 Hordeum-SxM-SSR-1H 163.6 Hv-CebadaCapa/SusPtrit-1H 158.35 Hv-Consensus2006-Marcel-1H 131.93 Hv-Integrated2009-1H 135.1 Hv-Nure/Tremois-1H 112.6
	[ Show Nearby Loci ]
Map Data	Barley, B73xCPI [ Show all 16 ]
Species	Hordeum spontaneum Hordeum vulgare
Probe	WMC1E8
Reference	Szucs P et al. (2009) An Integrated Resource for Barley Linkage Map and Malting Quality QTL Alignment The Plant Genome 2:134-140. [ Show all 6 ]
Remark	Number of populations contributing to the placement of WMC1E8 in the Barley, Consensus 2006 DArT maps = 1 [ Show all 8 ]
Probe	WMC1E8
Locus	WMC1E8
Reference	Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)24 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.43
Type	PCR SSR
PCR primers	5' TCATTCGTTGCAGATACACCAC 3' 5' TCAATGCCCTTGTTTCTGACCT 3' TCATTCGTTGCAGATACACCAC TCAATGCCCTTGTTTCTGACCT
SSR size	197
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.