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GrainGenes Marker Report: WMC1E8

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Locus	WMC1E8
Type	Microsatellite
Chromosome	1H
Map	Barley, B73xCPI-1H 127 [ Show all 16 ]
	[ Show Nearby Loci ]
Map Data	Barley, B73xCPI [ Show all 16 ]
Species	Hordeum spontaneum Hordeum vulgare
Probe	WMC1E8
Reference	Szucs P et al. (2009) An Integrated Resource for Barley Linkage Map and Malting Quality QTL Alignment The Plant Genome 2:134-140. [ Show all 6 ]
Remark	[ Hide all but 1 of 8 ] Number of populations contributing to the placement of WMC1E8 in the Barley, Consensus 2006 DArT maps = 1 Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = Steptoe x Morex,Oregon Wolfe Barleys,Cebada Capa x SusPtrit,Nure x Tremois Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : Steptoe x Morex; Oregon Wolfe Dom x Rec; Cebada Capa x SusPtrit Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Steptoe x Morex, OWBrecessive x OWBdominant, Igri x Franka, Lina x H. spontaneum Insert contains repeat (AC)24 Polymorphic Information Content (PIC) = 0.43 Developing laboratory = SCRI (R. Waugh)
Probe	WMC1E8
Locus	WMC1E8
Reference	Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AC)24 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.43
Type	PCR SSR
PCR primers	5' TCATTCGTTGCAGATACACCAC 3' 5' TCAATGCCCTTGTTTCTGACCT 3' TCATTCGTTGCAGATACACCAC TCAATGCCCTTGTTTCTGACCT
SSR size	197
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.