GrainGenes Marker Report: WMC215
Probe
WMC215
Locus
[ Hide all but 1 of 7 ] Xwmc215-5D Xwmc215 Xwmc215-3A Xwmc215-5A wmc215 wmc215-3A wmc215-5D
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 11e7 Donor: Benoist Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 211 bases located on 5D AGR205.5. Trimmed to Rsa1 at 5' end and to insert at 3' end. (GT)29 66 to 12.
Type
PCR SSR
PCR primers
Forward cATgcATggTTgcAAgcAAAAg at 16 length 22 bases, Tm 67
Reverse cATcccggTgcAAcATcTgAAA at 201 length 22 bases, Tm 69
SSR size
207 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC215
Source Species
Triticum aestivum
GrainGenes Marker Report: WMC215
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