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GrainGenes Marker Report: WMC219

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Probe	WMC219
Locus	[ Hide all but 1 of 7 ] Xwmc219-4A Xwmc219 Stb12 Yr60 SrND643 wmc219 wmc219-4A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease Resistance. Stripe Rust Resistance. Yr60 MAS Wheat. Marker Assisted Selection in Wheat.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11b9 Donor: Benoist AGR205.18. Overlap of forward and reverse is hypothetical - both end within a substantial micorsatellite. The fragment sizes for the PCR from this sequence will not match the expected size. Trimmed to Rsa1 at both ends. (CA)57 316 to 429.
Type	PCR SSR
PCR primers	Forward TgcTAgTTTgTcATccgggcgA at 264 length 22 bases, Tm 71 Reverse cAATcccgTTcTAcAAgTTccA at 446 length 22 bases, Tm 63
SSR size	~204 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC219
Source Gene	Yr60 (Triticum)
Source Species	Triticum aestivum