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GrainGenes Marker Report: WMC453

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Probe	WMC453
Locus	[ Hide all but 1 of 13 ] Xwmc453a-2A Xwmc453b-2B Xwmc453d-2D Xwmc453.2-2A Xwmc453.1-2B Xwmc453 Xwmc453-2A Xwmc453b Xwmc453a Xwmc453-2D wmc453 wmc453-2A wmc453-2D
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2c2 Donor: IGS BioTech Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 172 bases located on 2A Trimmed to insert at 5' end and to Rsa1 at 3' end. (CA)35 119 to 188.
Type	PCR SSR
PCR primers	Forward AcTTgTgTccATAAccgAccTT at 73 length 22 bases, Tm 61 Reverse ATcTTTTgAggTTAcAAcccgA at 238 length 22 bases, Tm 61
SSR size	187 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide (though complex).
Sequence	WMC453
Source Species	Triticum aestivum