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GrainGenes Marker Report: WMC473

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Probe	WMC473
Locus	[ Hide all but 1 of 9 ] Xwmc473-4D Xwmc473 Xwmc473a Xwmc473c Xwmc473-7D Xwmc473-6B wmc473 wmc473-6B wmc473-4D
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25e2 Donor: Jizeng, Beijing Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 111 bases located on 1D, 149 bases located on 4D Trimmed to Ssp1 at 5' end and to insert at 3' end. (CA)27 40 to 93.
Type	PCR SSR
PCR primers	Forward TcTgTTgcgcgAAAcAgAATAg at 6 length 22 bases, Tm 64 Reverse cccATTggAcAAcAcTTTcAcc at 122 length 22 bases, Tm 65
SSR size	138 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product. tested 51C anneal, no product.
Sequence	WMC473
Source Species	Triticum aestivum