GrainGenes Marker Report: WMC474
Probe
WMC474
Locus
[ Hide all but 1 of 7 ] Xwmc474-2B Xwmc474 Xwmc474b Xwmc474-2A wmc474 wmc474-2B wmc474-2A
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 25e3 Donor: Jizeng, Beijing Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 125 bases located on 2B Trimmed to insert at both ends. (GT)20 53 to 92.
Type
PCR SSR
PCR primers
Forward ATgcTATTAAAcTAgcATgTgTcg at 3 length 24 bases, Tm 60
Reverse AgTggAAAcATcATTccTggTA at 103 length 22 bases, Tm 60
SSR size
120 bases expected, Chinese Spring
Amplification Conditions
tested 61C anneal, no product. 51C anneal, product.
Sequence
WMC474
Source Species
Triticum aestivum
GrainGenes Marker Report: WMC474
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