GrainGenes Marker Report: WMC488
Probe
WMC488
Locus
[ Hide all but 1 of 7 ] Xwmc488b-7A Xwmc488a-7D Xwmc488-7A Xwmc488 Xwmc488-7D wmc488 wmc488-7A
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 25e8 Donor: Serasem Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 106 bases located on 7A Trimmed to insert at 5' end and to Ssp1 at 3' end. (GT)14 70 to 97, (GT)9 100 to 117.
Type
PCR SSR
PCR primers
Forward AAAgcAcAAccAgTTATgccAc at 39 length 22 bases, Tm 63
Reverse gAAccATAgTcAcATATcAcgAgg at 151 length 24 bases, Tm 62
SSR size
136 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic on acrylamide (120-140).
Sequence
WMC488
Source Species
Triticum aestivum
GrainGenes Marker Report: WMC488
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