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GrainGenes Marker Report: WMC505

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Locus	wmc505
Type	Microsatellite
Map	Durum Wheat, NSGC Lr 3A 52 Durum Wheat, NSGC Lr 3B 63.3 Physical-3B 56.7 Wheat, Yr genes and QTL 3A 27.1 Wheat, Yr genes and QTL 3B 37.5
	[ Show Nearby Loci ]
Map Data	3B Physical Durum Wheat, NSGC Lr Wheat, Yr genes and QTL
Probe	WMC505
Possible Orthologs	[ Hide all but 1 of 10 ] Xwmc505-3A Xwmc505-3B Xwmc505-3D Xwmc505.1 Xwmc505.2 Xwmc505a Xwmc505d wmc505-3A wmc505-3B Xwmc505
Probe	WMC505
Locus	Xwmc505-3A [ Show all 11 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 20g8 Donor: Byrne, Colorado State Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 115 bases located on 3A, 133 bases located on 3B, 90 bases located on 3D, 98 bases located on 3B No trimming done to sequence at Agrogene, ends not located. (CA)31 55 to 116.
Type	PCR SSR
PCR primers	Forward AggggAggAAAAccTTgTAATc at 28 length 22 bases, Tm 61 Reverse AcgAccTAcgTggTAgTTcTTg at 131 length 22 bases, Tm 60
SSR size	125 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide (several loci) .
Sequence	WMC505
Source Species	Triticum aestivum