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GrainGenes Marker Report: WMC51

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Probe	WMC51
Locus	[ Hide all but 1 of 17 ] Xwmc51-1B Xwmc51 Xwmc51g Xwmc51h Xwmc051 Xwmc51-2B Xwmc51-1A Xwmc51-3B Xwmc51-7B wmc51 wmc51-2B wmc51-4B wmc51-7B Xwmc51-4B wmc51-1B wmc51-1A wmc51-3B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6g5 Donor: Dubcovsky, Buenos Aires AGR87. Trimmed to insert. Interrupted (GT)12 microsatellite 259-272. (3 loci?).
Type	PCR SSR
PCR primers	Forward TTATcTTggTgTcTcATgTcAg at 230 length 22 bases, Tm 57 Reverse TcgcAAgATcATcAgAAcAgTA at 297 length 22 bases, Tm 60
SSR size	89 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC51
Source Species	Triticum aestivum