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GrainGenes Marker Report: WMS149

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Probe	WMS149
Locus	[ Hide all but 1 of 6 ] Xgwm149-4B Xgwm149 Xgwm149-4B.2 Xgwm149-4B.1 gwm149 gwm149-4B
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Remarks	Insert contains repeat (GA)23imp PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' CATTGTTTTCTGCCTCTAGCC 3' 5' CTAGCATCGAACCTGAACAAG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS149
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring