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GrainGenes Marker Report: WMS247

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Probe	WMS247
Locus	[ Hide all but 1 of 8 ] Xgwm247 Xgwm247-3A Xgwm247-3B Xgwm247.1-1B Xgwm247-6R gwm247 gwm247-3B gwm247-3A
Reference	Fu YB et al. (2007) Microsatellite variation in Avena sterilis oat germplasm Theoretical and Applied Genetics 114:1029-1038. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Remarks	Insert contains repeat (GA)24 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER. In 369 Avena sterilis accessions there were 4 alleles of size 39-60 bp, PIC = 0.04.
Type	Genomic PCR SSR
PCR primers	5' GCAATCTTTTTTCTGACCACG 3' 5' ATGTGCATGTCGGACGC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS247
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring