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GrainGenes Marker Report: WMS46

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Probe	WMS46
Locus	[ Hide all but 1 of 6 ] Xgwm46 Xgwm46-7B Xgwm46a Xgwm46b gwm46 gwm46-7B
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023. Roeder MS et al. (1995) Abundance, variability and chromosomal location of microsatellites in wheat. Molecular and General Genetics 246:327-333.
Remarks	Insert contains repeat (GA)2GC(GA)33 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER. Repeats type (GA)x2GC(GA)x33. Product size 187 bp in Chinese Spring.
Type	PCR SSR
PCR primers	5' GCACGTGAATGGATTGGAC 3' 5' TGACCCAATAGTGGTGGTCA 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS46
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring