GrainGenes Marker Report: WMS540
Probe
WMS540
Locus
[ Hide all but 1 of 7 ] Xgwm540-5B Xgwm540-4B Xgwm540 Xgwm540a gwm540 gwm540-4B gwm540-5B
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Remarks
Insert contains repeat (CT)3(CC)(CT)16 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' TCTCGCTGTGAAATCCTATTTC 3'
5' AGGCATGGATAGAGGGGC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS540
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: WMS540
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