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GrainGenes Marker Report: XHVM40g

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Locus
XHVM40g
Type
Microsatellite
Chromosome
5R
Chromosome Arm
5RS
Map
Secale-UxE-549.9
[ Show Nearby Loci ]
Map Data
Rye, UC90 x E-line
Probe
HVM40



Probe for Locus
HVM40
Locus
[ Hide all but 1 of 6 ]
HVM40
HVM0040
XHVM40a
XHVM40g
XHVM40e
HVM040
Reference
ReferenceHearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391.
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceLiu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
General Remarks
Insert contains repeats (GA)6, (GT)4, and (GA)7.
Type
PCR
SSR
PCR primers
5' CGATTCCCCTTTTCCCAC 3'
5' ATTCTCCGCCGTCCACTC 3'
CGATTCCCCTTTTCCCAC
ATTCTCCGCCGTCCACTC
SSR size
0.160
Amplification Conditions
The 'touchdown' PCR comprised 48 cycles of 94oC for 1 min denaturing and 72oC for 1 min extension. Annealing (30 s) temperatures were progressively decreased by 1oC every second cycle from 64oC to 55oC. Annealing conditions of 1 min at 55oC were maintained during the final 30 cycles. The reaction ended with a 5-min extension at 72oC.
Insert Enzyme
TaqI
Source Species
Hordeum vulgare
Source Germplasm
Old ZZ (barley)
DNA Origin
Size-fractionated genomic library screened with synthetic dinucleotide repeats (CA)10 and (GA)10.