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GrainGenes Marker Report: Xbarc74-5B

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Locus
Xbarc74-5B
Type
Microsatellite
Chromosome
5B
Map
Chinese_Spring_Deletion_SSR_5B0.42
[ Show all 7 ]
[ Show Nearby Loci ]
Breakpoint Interval
5BL6-0.29-0.55
Map Data
[ Hide all but 1 of 7 ]
Durum wheat, Kofa x UC1113
Wheat, Chinese Spring x SQ1
Wheat, Conan x Reeder
Wheat, Grandin x BR34
Wheat, Louise x Penawawa
Wheat, Physical, SSR
Wheat, Synthetic x Opata, BARC
Probe
BARC74
Possible Orthologs
Xbarc74
barc74-5B
barc74



Probe for Locus
BARC74
Locus
Xbarc74-5B
[ Show all 6 ]
General Remarks
Contains motif : (GA)13(GATA)7(GA)9
BARC primer pairs are developed by P. Cregan, Q. Song and associates at the USDA-ARS Beltsville Agriculture Research Station. Meiotic map information was developed by J.R. Shi, R. Ward and associates at Michigan State University. Deletion map information was developed by B.Gill, S. Singh and associates at Kansas State University. Funding for this research is provided by USDA-ARS in conjunction with the U.S. Wheat and Barley Scab Initiative.
Type
PCR
SSR
PCR primers
5' GCGCTTGCCCCTTCAGGCGAG 3'
5' CGCGGGAGAACCACCAGTGACAGAGC 3'
Amplification Conditions
Annealing temperature = 60
Sequence
BV211851
Source Species
Triticum aestivum