GrainGenes Marker Report: Xcfa2104-5D
Locus
Xcfa2104-5D
Type
Microsatellite
Chromosome
5D
Map
Ta-Courtot/CS-GPW-5D 28.3 Ta-SSR-2004-5D 13.7 USG3209/Jaypee-5D 55.1
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004 Wheat, Courtot x Chinese Spring, GPW Wheat, USG3209 x Jaypee
Probe
CFA2104
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
[ Hide all but 1 of 8 ] Xcfa2104-5A Xcfa2104b cfa2104 cfa2104a-5B cfa2104a cfa2104-5A cfa2104-5B cfa2104-5D
Probe for Locus
CFA2104
Locus
Xcfa2104-5A
[ Show all 9 ]
Reference
Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)13 Regularity of motif is imperfect
Type
PCR SSR
PCR primers
Forward 5' CCTGGCAGAGAAAGTGAAGG 3'
Reverse 5' AGTCGCCGTTGTATAGTGCC 3'
SSR size
294
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
5A/5D
Source Species
Triticum urartu
GrainGenes Marker Report: Xcfa2104-5D
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