GrainGenes Marker Report: Xcfa2141-5A
Locus
Xcfa2141-5A
Type
Microsatellite
Chromosome
5A
Chromosome Arm
5AL
Map
Chinese_Spring_Deletion_SSR_5A 0.825 T.b.xT.m.-5A 150.9 Ta-SSR-2004-5A 106.4 Ta-Synthetic/Opata-GPW-5A 135.3 Triticum-AxF-5A 136.4
[ Show Nearby Loci ]
Breakpoint Interval
5AL17-0.78-0.87
Map Data
T. boeoticum x monococcum Wheat, Arina x Forno Wheat, Consensus SSR, 2004 Wheat, Physical, SSR Wheat, Synthetic x Opata, GPW
Species
Triticum aestivum
Probe
CFA2141
Reference
Possible Orthologs
[ Hide all but 1 of 16 ] Xcfa2141 Xcfa2141-5D Xcfa2141a cfa2141 cfa2141b-5A cfa2141a-5A cfa2141b cfa2141_2 cfa2141a cfa2141_1 cfa2141-5A.1 cfa2141-5A.2 cfa2141-5A.3 cfa2141-5A.4 cfa2141-5D.1 cfa2141-5D.2
Probe for Locus
CFA2141
Locus
Xcfa2141-5A
[ Show all 17 ]
Reference
Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)18 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' GAATGGAAGGCGGACATAGA 3'
Reverse 5' GCCTCCACAACAGCCATAAT 3'
SSR size
229
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
5A/5D
Source Species
Triticum urartu
GrainGenes Marker Report: Xcfa2141-5A
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