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GrainGenes Marker Report: Xcfa2147c

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Locus	Xcfa2147c
Type	Microsatellite
Chromosome	1B
Map	Wheat-Composite2004-1B 97
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	CFA2147
Possible Orthologs	[ Hide all but 1 of 11 ] Xcfa2147-1A Xcfa2147-1B Xcfa2147-1B.1 Xcfa2147-1B.2 Xcfa2147-1D Xcfa2147a Xcfa2147b cfa2147 cfa2147-1A cfa2147-1B cfa2147-1D
Probe for Locus	CFA2147
Locus	Xcfa2147a [ Show all 12 ]
Reference	Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CATC)4 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' TCATCCCCTACATAACCCGA 3' Reverse 5' ATCGTGCACCAAGCAATACA 3'
SSR size	291
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	1B/1D/1B
Source Species	Triticum urartu