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GrainGenes Marker Report: Xcfd190

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Locus	Xcfd190
Type	Microsatellite
Chromosome	6A
Map	Wheat-Composite2004-6A 84
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	CFD190
Possible Orthologs	Xcfd190-6A [ Show all 7 ]
Probe for Locus	CFD190
Locus	[ Hide all but 1 of 8 ] Xcfd190-6A Xcfd0190-6B Xcfd190 Xcfd190-6D Xcfd190-6B cfd190 cfd190-6A cfd190-6D
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)20 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' CAATCAGAAGCGCCATTGTT 3' Reverse 5' CCCTGATGTTTTCTTTTTCTCC 3'
SSR size	191
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	6A
Source Species	Aegilops tauschii