Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Marker Report: Xcfd219-3D

[Submit comment/correction]

Locus	Xcfd219-3D
Type	Microsatellite
Map	Ta-Synthetic/Opata-GPW-3D 80.2
	[ Show Nearby Loci ]
Map Data	Wheat, Synthetic x Opata, GPW
Probe	CFD219
Possible Orthologs	[ Hide all but 1 of 6 ] Xcfd219-5B Xcfd219-6D Xcfd219a Xcfd219b cfd219 cfd219-6D
Probe for Locus	CFD219
Locus	Xcfd219-3D [ Show all 7 ]
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = C Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)13 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' GGCCCATCTGTCATTGACTT 3' Reverse 5' CAGCTTGTGTTGCTCGCTTA 3'
SSR size	296
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	5B/3D
Source Species	Aegilops tauschii