GrainGenes Marker Report: Xcfd283-5D
Locus
Xcfd283-5D
Type
Microsatellite
Chromosome
5D
Map
Louise/Penawawa-5D 187.2 Ta-SSR-2004-5D 90.2 Ta-Synthetic/Opata-GPW-5D 155.3
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004 Wheat, Louise x Penawawa Wheat, Synthetic x Opata, GPW
Probe
CFD283
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
[ Hide all but 1 of 7 ] cfd283 Xcfd283-3B Xcfd283-4B Xcfd283a cfd283-3B cfd283-4B cfd283-5D
Probe for Locus
CFD283
Locus
Xcfd283-4B
[ Show all 8 ]
Reference
General Remarks
Quality Score = D Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CCCA)3 Regularity of motif is imperfect
Type
PCR SSR
PCR primers
Forward 5' CCCGTGGTCTTGGGTTC 3'
Reverse 5' AGTTTTGCCATCGGCTGTAT 3'
SSR size
237
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
4B/5D
Source Species
Aegilops tauschii
GrainGenes Marker Report: Xcfd283-5D
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