GrainGenes Marker Report: Xgwm120-5A
Locus
Xgwm120-5A
Type
Microsatellite
Map
Kofa/UC1113-5A 26
[ Show Nearby Loci ]
Map Data
Durum wheat, Kofa x UC1113
Probe
WMS120
Possible Orthologs
[ Hide all but 1 of 8 ] Xgwm120 Xgwm120-2B gwm120b gwm120 gwm120-2B gwm120-2A gwm120-2B.1 gwm120-2B.2
Probe for Locus
WMS120
Locus
Xgwm120-2B
[ Show all 9 ]
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CT)11(CA)18 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' GATCCACCTTCCTCTCTCTC 3'
5' GATTATACTGGTGCCGAAAC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS120
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm120-5A
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