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GrainGenes Marker Report: Xgwm135

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Locus
Xgwm135
Type
Microsatellite
Chromosome
1A
Map
Ta-NxW-1A88.6
Wheat, UC1110 x PI610750 1A58.8
Wheat, WH542 x Synthetic 1A14.8
Wheat-Composite2004-1A54
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Wheat, Nanda2419 x Wangshuibai
Wheat, UC1110 x PI610750
Wheat, WH542 x Synthetic
Probe
WMS135
Reference
ReferenceLowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs
[ Hide all but 1 of 6 ]
Xgwm135-1A
Xgwm135-1A.1
Xgwm135-1A.2
gwm135
gwm135-1A
gwm135-1B



Probe for Locus
WMS135
Locus
Xgwm135-1A
[ Show all 7 ]
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (GA)20
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' TGTCAACATCGTTTTGAAAAGG 3'
5' ACACTGTCAACCTGGCAATG 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS135
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring