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GrainGenes Marker Report: Xgwm148-2B

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Locus
Xgwm148-2B
Type
Microsatellite
Chromosome
2B
Chromosome Arm
2BS
Map
Chinese_Spring_Deletion_SSR_2B-0.64
[ Show all 12 ]
[ Show Nearby Loci ]
Breakpoint Interval
2BS1-0.53-0.75
Map Data
[ Hide all but 1 of 12 ]
T. turgidum, JK x Cham1
T. turgidum, JK x Cham1, 2
T. turgidum, Messapia x dicoccoides, SSR
T. turgidum, Omrabi5 x dicoccoides, QTL
Wheat, Arina x Forno
Wheat, Chinese Spring x SQ1
Wheat, Consensus SSR, 2004
Wheat, Louise x Penawawa
Wheat, Physical, SSR
Wheat, Synthetic x Opata, BARC
Wheat, Synthetic x Opata, GPW
Wheat, Synthetic x Opata, SSR
Species
Triticum aestivum
Probe
WMS148
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
ReferencePaillard S et al. (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.) Theoretical and Applied Genetics 107:1235-1242.
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source
Roeder, Marion S.98.09.15
Remark
In Map Ta-Synthetic/Opata-SSR-2B, the intervals most likely to contain locus are Xmwg950 - Xtam72 or Xtam72 - Xcdo405
Possible Orthologs
gwm148
Xgwm148
gwm148-2B


Probe for Locus
WMS148
Locus
Xgwm148-2B
Xgwm148
Ne2
gwm148
gwm148-2B
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CA)22
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' GTGAGGCAGCAAGAGAGAAA 3'
5' CAAAGCTTGACTCAGACCAAA 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS148
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring