GrainGenes Marker Report: Xgwm159-5B
Locus
Xgwm159-5B
Type
Microsatellite
Chromosome
5B
Map
Chinese_Spring_Deletion_SSR_5B -0.215 Ta-SSR-2004-5B 56.8 Ta-Synthetic/Opata-BARC-5B 29 Ta-Synthetic/Opata-GPW-5B 25.6 Ta-Synthetic/Opata-SSR-5B 26.45
[ Show Nearby Loci ]
Breakpoint Interval
C-5BS4-0.43
Map Data
Wheat, Consensus SSR, 2004 Wheat, Physical, SSR Wheat, Synthetic x Opata, BARC Wheat, Synthetic x Opata, GPW Wheat, Synthetic x Opata, SSR
Probe
WMS159
Reference
Data Source
Roeder, Marion S. 98.09.15
Remark
In Map Ta-Synthetic/Opata-SSR-5B, the interval most likely to contain locus is between Xgwm544 and Xawm66
Possible Orthologs
[ Hide all but 1 of 6 ] Xgwm159-5D Xgwm159a Xgwm159b gwm159 Xgwm159 gwm159-5D
Probe for Locus
WMS159
Locus
Xgwm159-5B
[ Show all 7 ]
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (GT)15 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' GGGCCAACACTGGAACAC 3'
5' GCAGAAGCTTGTTGGTAGGC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS159
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm159-5B
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