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GrainGenes Marker Report: Xgwm16

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Locus
Xgwm16
Type
Microsatellite
Chromosome
2B
5D
7B
Map
Wheat-Composite2004-2B97
Wheat-Composite2004-5D21
Wheat-Composite2004-7B44
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Probe
WMS16
Possible Orthologs
Xgwm16-1A
[ Show all 8 ]



Probe for Locus
WMS16
Locus
[ Hide all but 1 of 9 ]
Xgwm16-2B
Xgwm16-7B
Xgwm16-5D
Xgwm16
gwm16
gwm16-2B
gwm16-5A
Xgwm16-1A
Xgwm16-5A
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (C)12ACAAA(CA)14(GA)18
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' GCTTGGACTAGCTAGAGTATCATAC 3'
5' CAATCTTCAATTCTGTCGCACGG 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 50 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS16
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring