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GrainGenes Marker Report: Xgwm165-4A

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Locus	Xgwm165-4A
Type	Microsatellite
Chromosome	4A
Chromosome Arm	4AS
Map	[ Hide all but 1 of 8 ] Chinese_Spring_Deletion_SSR_4A -0.88 Kofa/UC1113-4A 0 Ta-Courtot/CS-GPW-4A 34.1 Ta-SSR-2004-4A 2.4 Ta-Synthetic/Opata-BARC-4A 17.9 Ta-Synthetic/Opata-GPW-4A 19.4 Ta-Synthetic/Opata-SSR-4A 12.2 Tt-Oxd-QTL-4A 0
	[ Show Nearby Loci ]
Breakpoint Interval	4AS3-0.76-1.00
Map Data	Durum wheat, Kofa x UC1113 [ Show all 8 ]
Probe	WMS165
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source	Roeder, Marion S. 98.09.15
Remark	In Map Ta-Synthetic/Opata-SSR-4A, the intervals most likely to contain locus are Xfba320 - XksuF8 or distal to XksuF8
Possible Orthologs	Xgwm165-4B [ Show all 13 ]
Probe for Locus	WMS165
Locus	Xgwm165.3-4A [ Show all 14 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)20 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' TGCAGTGGTCAGATGTTTCC 3' 5' CTTTTCTTTCAGATTGCGCC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS165
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring