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GrainGenes Marker Report: Xgwm182-5D

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Locus	Xgwm182-5D
Type	Microsatellite
Chromosome	5D
Chromosome Arm	5DL
Map	Chinese_Spring_Deletion_SSR_5D 0.67 [ Show all 9 ]
	[ Show Nearby Loci ]
Breakpoint Interval	5DL1-0.60-0.74
Map Data	[ Hide all but 1 of 9 ] Wheat, Conan x Reeder Wheat, Consensus SSR, 2004 Wheat, Grandin x BR34 Wheat, Louise x Penawawa Wheat, Physical, SSR Wheat, Synthetic x Opata, BARC Wheat, Synthetic x Opata, GPW Wheat, Synthetic x Opata, SSR Wheat, Synthetic x Opata, SSR 2
Probe	WMS182
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source	Roeder, Marion S. 98.09.15
Possible Orthologs	Xgwm182 Xgwm182-6B gwm182 gwm182-5D
Probe for Locus	WMS182
Locus	Xgwm182-5D Xgwm182-6B Xgwm182 gwm182 gwm182-5D
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CT)18 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' TGATGTAGTGAGCCCATAGGC 3' 5' TTGCACACAGCCAAATAAGG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS182
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring