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GrainGenes Marker Report: Xgwm234

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Locus
Xgwm234
Type
Microsatellite
Chromosome
5B
Chromosome Arm
5BS
Map
Svevo/Ciccio-5B0
Ta-NxW-5B12.5
Tt-LxG-5B0
Wheat, Avalon x Cadenza 5B112.9
Wheat-Composite2004-5B21
[ Show Nearby Loci ]
Breakpoint Interval
5BS6-0.81-1.00
Map Data
Durum wheat, Svevo x Ciccio
T. turgidum, Langdon x G18-16
Wheat, Avalon x Cadenza
Wheat, Composite, 2004
Wheat, Nanda2419 x Wangshuibai
Probe
WMS234
Possible Orthologs
[ Hide all but 1 of 9 ]
Xgwm234-5A
Xgwm234-5B
gwm234
gwm234a-5B
gwm234b-5B
gwm234b
gwm234-5B
gwm234-5B.1
gwm234-5B.2



Probe for Locus
WMS234
Locus
Xgwm234-5B
[ Show all 10 ]
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CT)16(CA)20
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' GAGTCCTGATGTGAAGCTGTTG 3'
5' CTCATTGGGGTGTGTACGTG 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS234
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring