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GrainGenes Marker Report: Xgwm269

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Locus
Xgwm269
Type
Microsatellite
Chromosome
5D
7R
Chromosome Arm
7RS
Map
Rye-2009-Consensus-7R5.2
Secale-PxP-update-7R5.4
Wheat, UC1110 x PI610750 5D201.9
Wheat-Composite2004-5D99
[ Show Nearby Loci ]
Map Data
Rye, P87 x P105, update
Rye_2009_consensus
Wheat, Composite, 2004
Wheat, UC1110 x PI610750
Probe
WMS269
Reference
ReferenceLowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs
Xgwm269-5D
[ Show all 7 ]



Probe for Locus
WMS269
Locus
[ Hide all but 1 of 8 ]
Xgwm269.2-4A
Xgwm269.1-5D
Xgwm269-5D
Xgwm269
Xgwm269.2
Xgwm269-7R
gwm269
gwm269-5D
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CA)29
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' TGCATATAAACAGTCACACACCC 3'
5' TTTGAGCTCCAAAGTGAGTTAGC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS269
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring