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GrainGenes Marker Report: Xgwm271a

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Locus	Xgwm271a
Type	Microsatellite
Chromosome	5B
Map	Wheat-Composite2004-5B 101
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	WMS271
Possible Orthologs	Xgwm271 [ Show all 14 ]
Probe for Locus	WMS271
Locus	[ Hide all but 1 of 16 ] Xgwm271-5B Xgwm271-5D Xgwm271 Sr36 Xgwm271-2B Xgwm271a Xgwm271b Xgwm271-5A gwm271 gwm271-5B gwm271a gwm271a-2B gwm271b gwm271b-5A gwm271-2B.1 gwm271-2B.2
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CT)4imp(GA)10 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' CAAGATCGTGGAGCCAGC 3' 5' AGCTGCTAGCTTTTGGGACA 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS271
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring