GrainGenes Marker Report: Xgwm274-5B
Locus
Xgwm274-5B
Type
Microsatellite
Chromosome
5B
Chromosome Arm
5BS
Map
Ta-Courtot/CS-GPW-5B 15.9 Ta-SSR-2004-5B 63.6 Triticum-AxF-5B 31
[ Show Nearby Loci ]
Map Data
Wheat, Arina x Forno Wheat, Consensus SSR, 2004 Wheat, Courtot x Chinese Spring, GPW
Species
Triticum aestivum
Probe
WMS274
Reference
Possible Orthologs
gwm274
[ Show all 14 ]
Probe for Locus
WMS274
Locus
[ Hide all but 1 of 15 ] Xgwm274-1B Xgwm274-5B Xgwm274-7B Xgwm274.2 Xgwm274.1 Xgwm274a Xgwm274 Xgwm274-3B gwm274 gwm274-1B gwm274-7B Xgwm274a-4A Xgwm274b gwm274-3B gwm274-5B
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (GT)27 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' AACTTGCAAAACTGTTCTGA 3'
5' TATTTGAAGCGGTTTGATTT 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 50 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS274
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm274-5B
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