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GrainGenes Marker Report: Xgwm296-2A

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Locus	Xgwm296-2A
Type	Microsatellite
Chromosome	2A
Chromosome Arm	2AS
Map	[ Hide all but 1 of 7 ] Chinese_Spring_Deletion_SSR_2A -0.39 Kofa/UC1113-2A 22.8 Ta-SSR-2004-2A 13.3 Ta-Synthetic/Opata-BARC-2A 43.6 Ta-Synthetic/Opata-GPW-2A 56.7 Ta-Synthetic/Opata-SSR-2A 8.45 Tturgidum-SSR-2A -85.9
	[ Show Nearby Loci ]
Breakpoint Interval	C-2AS5-0.78
Map Data	Durum wheat, Kofa x UC1113 [ Show all 7 ]
Probe	WMS296
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source	Roeder, Marion S. 98.09.15
Remark	In Map Ta-Synthetic/Opata-SSR-2A, the intervals most likely to contain locus are Xgwm614 - Xbcd348 or Xbcd348 - Xcdo57
Possible Orthologs	Xgwm296 [ Show all 8 ]
Probe for Locus	WMS296
Locus	Xgwm296-7D [ Show all 10 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CT)28 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' AATTCAACCTACCAATCTCTG 3' 5' GCCTAATAAACTGAAAACGAG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS296
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring