GrainGenes Marker Report: Xgwm299-2B
Locus
Xgwm299-2B
Type
Microsatellite
Chromosome
2B
Chromosome Arm
2BS
Map
Tt-Oxd-QTL-2B 100.8
[ Show Nearby Loci ]
Map Data
T. turgidum, Omrabi5 x dicoccoides, QTL
Probe
WMS299
Possible Orthologs
gwm299 Xgwm299 Xgwm299-3B Xgmw299 gwm299-3B
Probe for Locus
WMS299
Locus
[ Hide all but 1 of 6 ] Xgwm299-3B Xgwm299 Xgwm299-2B gwm299 gwm299-3B Xgmw299
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (GA)31(TAG)4 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' ACTACTTAGGCCTCCCGCC 3'
5' TGACCCACTTGCAATTCATC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS299
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm299-2B
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