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GrainGenes Marker Report: Xgwm314-3D

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Locus	Xgwm314-3D
Type	Microsatellite
Chromosome	3D
Chromosome Arm	3DL
Map	Chinese_Spring_Deletion_SSR_3D 0.905 [ Show all 9 ]
	[ Show Nearby Loci ]
Breakpoint Interval	3DL3-0.81-1.00
Map Data	[ Hide all but 1 of 9 ] Wheat, Conan x Reeder Wheat, Consensus SSR, 2004 Wheat, Courtot x Chinese Spring, GPW Wheat, Physical, SSR Wheat, Sphaerococcoid Genes Wheat, Synthetic x Opata, BARC Wheat, Synthetic x Opata, GPW Wheat, Synthetic x Opata, SSR Wheat, Synthetic x Opata, update
Probe	WMS314
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Salina E et al. (2000) Microsatellite mapping of the induced sphaerococcoid mutation genes in Triticum aestivum Theoretical and Applied Genetics 100:686-689. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source	Roeder, Marion S. 98.09.15
Remark	In Map Ta-Synthetic/Opata-SSR-3D, the interval most likely to contain locus is between XksuH15 and Xbcd361
Possible Orthologs	Xgwm314 Xgwm314-4B gwm314 gwm314-3D
Probe for Locus	WMS314
Locus	Xgwm314-3D Xgwm314-4B Xgwm314 gwm314 gwm314-3D
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CT)25imp PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' AGGAGCTCCTCTGTGCCAC 3' 5' TTCGGGACTCTCTTCCCTG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS314
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring