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GrainGenes Marker Report: Xgwm328b

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Locus
Xgwm328b
Type
Microsatellite
Chromosome
2A
Map
Wheat-Composite2004-2A64
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Probe
WMS328
Possible Orthologs
Xgwm328
Xgwm328-2A
Xgwm328a
gwm328-2A
gwm328



Probe for Locus
WMS328
Locus
[ Hide all but 1 of 6 ]
Xgwm328-2A
Xgwm328
Xgwm328a
Xgwm328b
gwm328
gwm328-2A
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (GT)14
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' GCAATCCACGAGAAGAGAGG 3'
5' CACAAACTCTTGACATGTGCG 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS328
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring