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GrainGenes Marker Report: Xgwm335-5B

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Locus	Xgwm335-5B
Type	Microsatellite
Chromosome	5B
Chromosome Arm	5BL
Map	Chinese_Spring_Deletion_SSR_5B 0.42 [ Show all 10 ]
	[ Show Nearby Loci ]
Breakpoint Interval	5BL6-0.29-0.55
Map Data	[ Hide all but 1 of 10 ] Durum wheat, Kofa x UC1113 T. turgidum, Omrabi5 x dicoccoides, QTL Wheat, Arina x Forno Wheat, Consensus SSR, 2004 Wheat, Louise x Penawawa Wheat, P91193D1 x P92201D5 Wheat, Physical, SSR Wheat, Synthetic x Opata, BARC Wheat, Synthetic x Opata, GPW Wheat, Synthetic x Opata, SSR
Species	Triticum aestivum
Probe	WMS335
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Paillard S et al. (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.) Theoretical and Applied Genetics 107:1235-1242. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source	Roeder, Marion S. 98.09.15
Possible Orthologs	Xgwm335 Xgwm335-5R gwm335 gwm335-5B
Probe for Locus	WMS335
Locus	Xgwm335-5B Xgwm335 Xgwm335-5R gwm335 gwm335-5B
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)14(GCGT)3 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' CGTACTCCACTCCACACGG 3' 5' CGGTCCAAGTGCTACCTTTC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS335
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring