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GrainGenes Marker Report: Xgwm459

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Locus	Xgwm459
Type	Microsatellite
Map	Ta-NxW-6A 10 Wheat_Yr81_2019-6A 12.5
	[ Show Nearby Loci ]
Map Data	T. boeoticum x monococcum Wheat, Nanda2419 x Wangshuibai Wheat_Yr81_2019
Probe	WMS459
Possible Orthologs	Xgwm459-6A Xgwm459.1 Xgwm459.2 gwm459 gwm459-6A
Probe for Locus	WMS459
Locus	[ Hide all but 1 of 6 ] Xgwm459-6A Xgwm459 Xgwm459.1 Xgwm459.2 gwm459 gwm459-6A
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)>28 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' ATGGAGTGGTCACACTTTGAA 3' 5' AGCTTCTCTGACCAACTTCTCG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS459
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring