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GrainGenes Marker Report: Xgwm47-2A.1

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Locus
Xgwm47-2A.1
Type
Microsatellite
Chromosome
2A
Chromosome Arm
2AL
Map
Ta-Synthetic/Opata-BARC-2A86.2
Ta-Synthetic/Opata-GPW-2A130.9
Ta-Synthetic/Opata-SSR-2A113.8
Tturgidum-JKxC-2A6
Tturgidum-JKxC2-2A34.8
[ Show Nearby Loci ]
Map Data
T. turgidum, JK x Cham1
[ Show all 6 ]
Probe
WMS47
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source
Roeder, Marion S.98.09.15
Remark
In Map Ta-Synthetic/Opata-SSR-2A, the intervals most likely to contain locus are Xbcd543 - XksuE16 or XksuE16 - Xpsr934
Possible Orthologs
gwm47
[ Show all 14 ]



Probe for Locus
WMS47
Locus
[ Hide all but 1 of 16 ]
Xgwm47-2A.2
Xgwm47-2A.1
Xgwm47-2B
Xgwm47-5A
Xgwm47.3
Xgwm47.1
Xgwm47.4
Xgwm47.2
Xgwm47b
Xgwm47
Xgwm47-2A
Sr9a
gwm47
gwm47-2A
gwm47-2B
gwm47-5A
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
ReferenceRoeder MS et al. (1995) Abundance, variability and chromosomal location of microsatellites in wheat. Molecular and General Genetics 246:327-333.
General Remarks
Insert contains repeat (CT)7TT(CT)16
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Repeats type (CT)x7TT(CT)x16.
Product size 166 bp in Chinese Spring.
Type
PCR
SSR
PCR primers
5' TTGCTACCATGCATGACCAT 3'
5' TTCACCTCGATTGAGGTCCT 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS47
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring