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GrainGenes Marker Report: Xgwm47b

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Locus	Xgwm47b
Type	Microsatellite
Chromosome	2A
Map	Wheat-Composite2004-2A 83
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	WMS47
Possible Orthologs	gwm47 [ Show all 14 ]
Probe for Locus	WMS47
Locus	[ Hide all but 1 of 16 ] Xgwm47-2A.2 Xgwm47-2A.1 Xgwm47-2B Xgwm47-5A Xgwm47.3 Xgwm47.1 Xgwm47.4 Xgwm47.2 Xgwm47b Xgwm47 Xgwm47-2A Sr9a gwm47 gwm47-2A gwm47-2B gwm47-5A
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023. Roeder MS et al. (1995) Abundance, variability and chromosomal location of microsatellites in wheat. Molecular and General Genetics 246:327-333.
General Remarks	Insert contains repeat (CT)7TT(CT)16 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER. Repeats type (CT)x7TT(CT)x16. Product size 166 bp in Chinese Spring.
Type	PCR SSR
PCR primers	5' TTGCTACCATGCATGACCAT 3' 5' TTCACCTCGATTGAGGTCCT 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS47
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring