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GrainGenes Marker Report: Xgwm480-3A

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Locus	Xgwm480-3A
Type	Microsatellite
Chromosome	3A
Chromosome Arm	3AL 3AS
Map	Chinese_Spring_Deletion_SSR_3A 0.21 [ Show all 9 ]
	[ Show Nearby Loci ]
Breakpoint Interval	C-3AL3-0.42
Map Data	[ Hide all but 1 of 9 ] T.dicoccoides, FHB QTL Wheat, Chinese Spring x SQ1 Wheat, Consensus SSR, 2004 Wheat, P91193D1 x P92201D5 Wheat, Physical, SSR Wheat, Sphaerococcoid Genes Wheat, Synthetic x Opata, BARC Wheat, Synthetic x Opata, SSR Wheat, Synthetic x Opata, update
Probe	WMS480
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Otto CD et al. (2002) Genetic dissection of a major Fusarium head blight QTL in tetraploid wheat Plant Molecular Biology 48:625-632. Salina E et al. (2000) Microsatellite mapping of the induced sphaerococcoid mutation genes in Triticum aestivum Theoretical and Applied Genetics 100:686-689. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source	Roeder, Marion S. 98.09.15
Remark	In Map Ta-Synthetic/Opata-SSR-3A, the interval most likely to contain locus is between Xmwg30 and Xgwm162
Possible Orthologs	Xgwm480 gwm480 gwm480-3A
Probe for Locus	WMS480
Locus	Xgwm480-3A Xgwm480 gwm480 gwm480-3A
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CT)16(CA)13 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' TGCTGCTACTTGTACAGAGGAC 3' 5' CCGAATTGTCCGCCATAG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS480
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring