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GrainGenes Marker Report: Xgwm494b

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Locus
Xgwm494b
Type
Microsatellite
Chromosome
5D
6A
Map
Wheat-Composite2004-5D39
Wheat-Composite2004-6A96
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Probe
WMS494
Possible Orthologs
[ Hide all but 1 of 14 ]
Xgwm494
Xgwm494-1B
Xgwm494-3A
Xgwm494-4A
Xgwm494-5D
Xgwm494-6A
Xgwm494-6A.1
Xgwm494-6A.2
Xgwm494a
gwm494
gwm494-1B
gwm494-3A
gwm494-4A
gwm494-6A



Probe for Locus
WMS494
Locus
Xgwm494-4A
[ Show all 15 ]
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CA)13
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' ATTGAACAGGAAGACATCAGGG 3'
5' TTCCTGGAGCTGTCTGGC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS494
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring