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GrainGenes Marker Report: Xgwm497

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Locus	Xgwm497
Type	Microsatellite
Map	Svevo/Ciccio-1A 189.4
	[ Show Nearby Loci ]
Breakpoint Interval	1AL3-0.61-1.00
Map Data	Durum wheat, Svevo x Ciccio
Probe	WMS497
Possible Orthologs	[ Hide all but 1 of 22 ] Xgwm497-1A Xgwm497-2A Xgwm497-3A Xgwm497-3D Xgwm497-5B Xgwm497.1 Xgwm497.1-1A Xgwm497.2 Xgwm497.2-1A Xgwm497.2-5B Xgwm497.3 Xgwm497.3-3A Xgwm497.4 Xgwm497.4-5B Xgwm497.5 Xgwm497a Xgwm497b gwm497 gwm497-1A gwm497-3A gwm497-5B gwm497-2A
Probe for Locus	WMS497
Locus	Xgwm497.2-1A [ Show all 23 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GT)29imp PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' GTAGTGAAGACAAGGGCATT 3' 5' CCGAAAGTTGGGTGATATAC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS497
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring