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GrainGenes Marker Report: Xgwm501-2B

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Locus	Xgwm501-2B
Type	Microsatellite
Chromosome	2B
Map	[ Hide all but 1 of 7 ] Chinese_Spring_Deletion_SSR_2B 0.945 Conan/Reeder-2B 144.92 Louise/Penawawa-2B 6 Ta-SSR-2004-2B 85.4 Ta-Synthetic/Opata-BARC-2B 55 Ta-Synthetic/Opata-GPW-2B 82.9 Ta-Synthetic/Opata-SSR-2B 134.85
	[ Show Nearby Loci ]
Breakpoint Interval	2BL6-0.89-1.00
Map Data	Wheat, Conan x Reeder [ Show all 7 ]
Probe	WMS501
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source	Roeder, Marion S. 98.09.15
Remark	In Map Ta-Synthetic/Opata-SSR-2B, the interval most likely to contain locus is between Xgwm16 and Xbcd307
Possible Orthologs	Xgwm501 [ Show all 6 ]
Probe for Locus	WMS501
Locus	Xgwm501-2B [ Show all 8 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CA)33 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' GGCTATCTCTGGCGCTAAAA 3' 5' TCCACAAACAAGTAGCGCC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS501
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring