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GrainGenes Marker Report: Xgwm512-2A

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Locus	Xgwm512-2A
Type	Microsatellite
Chromosome	2A
Chromosome Arm	2AS
Map	Chinese_Spring_Deletion_SSR_2A -0.39 [ Show all 7 ]
	[ Show Nearby Loci ]
Breakpoint Interval	C-2AS5-0.78
Map Data	[ Hide all but 1 of 7 ] T. turgidum, Omrabi5 x dicoccoides, QTL Wheat, Consensus SSR, 2004 Wheat, P91193D1 x P92201D5 Wheat, Physical, SSR Wheat, Synthetic x Opata, BARC Wheat, Synthetic x Opata, GPW Wheat, Synthetic x Opata, SSR
Probe	WMS512
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source	Roeder, Marion S. 98.09.15
Remark	In Map Ta-Synthetic/Opata-SSR-2A, the interval most likely to contain locus is between XksuD18 and Xgwm359
Possible Orthologs	Xgwm512 gwm512
Probe for Locus	WMS512
Locus	Xgwm512-2A Xgwm512 gwm512
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GT)16 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' AGCCACCATCAGCAAAAATT 3' 5' GAACATGAGCAGTTTGGCAC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS512
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring